Libraries
require(pacman)
pacman::p_load(tidyverse, janitor, here, DT, gt, phylotools, assertr, readxl, patchwork, reshape2, ggtext, openxlsx, grid, tiff)
Load Data
virus_master_2023 <- read_csv(here("Data/tblastx_master.csv"))
BLAST Summary
library_summary <- virus_master_2023 %>%
group_by(mosquito_species, sample_number) %>%
summarise(n = n_distinct(mosquito_species)) %>%
group_by(mosquito_species) %>%
summarise(n = n()) %>%
adorn_totals()
years <- virus_master_2023 %>%
group_by(collection_year, virus_name) %>%
summarise(count = n_distinct(virus_name, collection_year)) %>%
pivot_wider(names_from = collection_year, values_from = count, values_fill = 0)
virus_lineage <- virus_master_2023 %>%
group_by(virus_name, viral_family, genome) %>%
summarise(n = n_distinct(virus_name, viral_family, genome)) %>%
select(-"n")
virus_master_2023 %>%
group_by(virus_name) %>%
summarise(n_contigs = n(),
mean_cov = mean(coverage),
min_cov = min(coverage),
max_cov = max(coverage),
mean_pid = mean(greatest_identity_percent),
med_pid = median(greatest_identity_percent),
min_pid = min(greatest_identity_percent),
max_pid = max(greatest_identity_percent),
total_reads = sum(total_read_count)) %>%
left_join(virus_lineage, by = "virus_name") %>%
group_by(genome, viral_family) %>%
arrange(genome, viral_family) %>%
relocate(total_reads, .after = "n_contigs") %>%
mutate(across(.cols = "virus_name",
~case_when(grepl("Manitoba", virus_name) & !grepl("Manitoba virus", virus_name) ~
paste0(., "*"),
TRUE ~ .))) %>%
gt() %>%
fmt_number(columns = mean_cov:max_pid,
decimals = 2) %>%
fmt_number(columns = total_reads,
sep_mark = ",",
decimals = 0) %>%
tab_spanner(label = "Coverage Depth", columns = c(mean_cov, min_cov, max_cov)) %>%
tab_spanner(label = "aa Percent Identity", columns = c(mean_pid, min_pid, max_pid, med_pid)) %>%
cols_label(
n_contigs = "Contigs",
total_reads = "Reads",
mean_cov = "Mean", min_cov = "Min", max_cov = "Max",
mean_pid = "Mean", min_pid = "Min", max_pid = "Max", med_pid = "Median",
virus_name = "Virus") %>%
tab_style(
style = list(cell_fill(color = "grey"),
cell_text(weight = "bold")),
locations = cells_row_groups(groups = everything())
) %>%
tab_style(
style = cell_borders(
sides = "left",
weight = px(2),
color = "grey"),
locations = cells_body(
columns = c(mean_cov, mean_pid, "n_contigs")
)
) %>%
data_color(
columns = mean_pid:max_pid,
palette = "viridis"
) %>%
tab_footnote("* Putatively novel virus.")
| Virus |
Contigs |
Reads |
Coverage Depth
|
aa Percent Identity
|
| Mean |
Min |
Max |
Mean |
Min |
Max |
Median |
| +ssRNA - Dicistroviridae |
| Black queen cell virus |
2 |
1,613 |
17.00 |
11.15 |
22.85 |
100.00 |
100.00 |
100.00 |
100.00 |
| Manitoba dicistro-like virus 1* |
1 |
10,688 |
149.27 |
149.27 |
149.27 |
82.61 |
82.61 |
82.61 |
82.61 |
| Soybean thrips dicistrovirus |
11 |
11,192 |
17.32 |
10.11 |
29.01 |
100.00 |
100.00 |
100.00 |
100.00 |
| +ssRNA - Flaviviridae |
| Inari jingmenvirus |
1 |
139 |
11.02 |
11.02 |
11.02 |
100.00 |
100.00 |
100.00 |
100.00 |
| Placeda virus |
169 |
106,682 |
90.37 |
10.02 |
1,114.82 |
97.87 |
86.24 |
100.00 |
98.55 |
| +ssRNA - Iflaviridae |
| Cafluga virus |
2 |
976 |
20.82 |
19.41 |
22.24 |
99.77 |
99.55 |
100.00 |
99.77 |
| Culex Iflavi-like virus 4 |
23 |
13,815 |
22.81 |
10.65 |
58.90 |
99.81 |
96.20 |
100.00 |
100.00 |
| Culex iflavilike virus 3 |
183 |
266,451 |
284.65 |
18.65 |
1,269.06 |
99.49 |
94.19 |
100.00 |
100.00 |
| Hanko iflavirus 1 |
30 |
2,938,402 |
3,394.01 |
10.99 |
19,795.35 |
97.49 |
93.45 |
100.00 |
97.30 |
| Hanko iflavirus 2 |
29 |
29,456 |
86.71 |
16.30 |
242.52 |
88.70 |
67.08 |
100.00 |
89.58 |
| Hubei arthropod virus 1 |
1 |
352 |
11.95 |
11.95 |
11.95 |
100.00 |
100.00 |
100.00 |
100.00 |
| Manitoba iflavirus 1* |
25 |
39,865 |
28.41 |
10.35 |
266.71 |
93.47 |
89.14 |
100.00 |
93.38 |
| Manitoba picorna-like virus 1* |
86 |
72,405 |
64.99 |
10.27 |
207.10 |
87.73 |
73.93 |
100.00 |
87.80 |
| Soybean thrips iflavirus 4 |
1 |
636 |
16.60 |
16.60 |
16.60 |
100.00 |
100.00 |
100.00 |
100.00 |
| Yongsan picorna-like virus 2 |
4 |
58,132 |
165.26 |
34.98 |
535.91 |
100.00 |
100.00 |
100.00 |
100.00 |
| +ssRNA - Luteoviridae |
| Marma virus |
23 |
39,344 |
70.95 |
11.62 |
129.64 |
100.00 |
100.00 |
100.00 |
100.00 |
| +ssRNA - Narnaviridae |
| Culex narnavirus 1 |
1 |
63 |
12.07 |
12.07 |
12.07 |
100.00 |
100.00 |
100.00 |
100.00 |
| Manitoba narnavirus 1* |
2 |
391 |
18.77 |
13.02 |
24.52 |
80.59 |
80.29 |
80.89 |
80.59 |
| +ssRNA - Negevirus |
| Cordoba virus |
14 |
135,594 |
548.17 |
17.51 |
2,225.85 |
97.10 |
93.75 |
100.00 |
97.23 |
| Manitoba mononega-like virus 1* |
3 |
12,701 |
45.19 |
33.85 |
59.07 |
80.60 |
73.21 |
90.00 |
78.57 |
| Manitoba mononega-like virus 2* |
3 |
6,079 |
37.12 |
18.89 |
65.64 |
98.77 |
96.30 |
100.00 |
100.00 |
| Mekrijarvi Negevirus |
6 |
167,549 |
294.01 |
30.28 |
1,262.63 |
94.13 |
90.78 |
100.00 |
93.40 |
| Serbia mononega-like virus 1 |
1 |
494 |
20.03 |
20.03 |
20.03 |
75.00 |
75.00 |
75.00 |
75.00 |
| Utsjoki negevirus 3 |
2 |
416 |
21.12 |
18.00 |
24.24 |
99.02 |
99.01 |
99.03 |
99.02 |
| +ssRNA - Nodaviridae |
| Hubei noda-like virus 12 |
2 |
4,244 |
54.47 |
23.60 |
85.34 |
95.83 |
91.67 |
100.00 |
95.83 |
| +ssRNA - Rhabdoviridae |
| Manitoba Rhabdovirus 1* |
37 |
32,534 |
34.99 |
10.21 |
120.41 |
83.22 |
69.42 |
93.01 |
83.33 |
| +ssRNA - Tombusviridae |
| Des Moines River virus |
4 |
35,117 |
486.06 |
34.94 |
897.17 |
100.00 |
100.00 |
100.00 |
100.00 |
| Hubei mosquito virus 4 |
6 |
2,919 |
16.68 |
10.02 |
39.59 |
96.50 |
92.65 |
100.00 |
95.70 |
| Manitoba tombus-like virus 1* |
6 |
28,922 |
224.21 |
74.52 |
557.41 |
79.73 |
72.73 |
100.00 |
76.56 |
| +ssRNA - Tymoviridae |
| Hubei macula-like virus 3 |
8 |
530,385 |
1,092.52 |
11.15 |
7,749.86 |
96.13 |
92.15 |
100.00 |
96.63 |
| Manitoba tymo-like virus 1* |
1 |
1,482 |
23.67 |
23.67 |
23.67 |
66.79 |
66.79 |
66.79 |
66.79 |
| +ssRNA - Virgaviridae |
| Hubei virga-like virus 2 |
6 |
505 |
13.26 |
10.94 |
15.50 |
99.04 |
96.27 |
100.00 |
100.00 |
| Manitoba virgavirus 1* |
1 |
186 |
11.06 |
11.06 |
11.06 |
83.83 |
83.83 |
83.83 |
83.83 |
| -ssRNA - Chuviridae |
| Chuvirus |
39 |
22,130 |
28.00 |
10.17 |
214.52 |
97.49 |
89.66 |
100.00 |
97.02 |
| -ssRNA - Culex rhabdovirus |
| Culex rhabdovirus |
2 |
997 |
116.26 |
74.47 |
158.06 |
100.00 |
100.00 |
100.00 |
100.00 |
| -ssRNA - Flanders hapavirus |
| Flanders hapavirus |
81 |
104,235 |
113.05 |
10.00 |
686.87 |
99.90 |
95.96 |
100.00 |
100.00 |
| -ssRNA - Orthomyxoviridae |
| Astopletus virus |
29 |
5,975 |
32.63 |
10.91 |
107.95 |
99.06 |
95.77 |
100.00 |
100.00 |
| Wuhan mosquito virus 6 |
101 |
133,667 |
176.35 |
12.58 |
641.58 |
99.76 |
97.69 |
100.00 |
100.00 |
| -ssRNA - Peribunyaviridae |
| Culex bunyavirus 2 |
19 |
6,255 |
23.92 |
10.28 |
49.84 |
100.00 |
100.00 |
100.00 |
100.00 |
| -ssRNA - Rhabdoviridae |
| Canya virus |
14 |
3,766 |
24.71 |
11.30 |
66.33 |
87.32 |
66.04 |
100.00 |
85.07 |
| Culex Rhabdo-like virus |
4 |
807 |
14.68 |
10.87 |
23.28 |
100.00 |
100.00 |
100.00 |
100.00 |
| Elisy virus |
21 |
3,849 |
19.29 |
10.10 |
40.69 |
99.27 |
96.41 |
100.00 |
100.00 |
| Manitoba rhabdovirus 2* |
1 |
2,401 |
51.70 |
51.70 |
51.70 |
75.00 |
75.00 |
75.00 |
75.00 |
| Manitoba virus |
5 |
1,003 |
19.71 |
15.74 |
24.80 |
99.76 |
98.80 |
100.00 |
100.00 |
| Merida virus |
55 |
76,697 |
91.60 |
11.06 |
276.87 |
99.86 |
97.14 |
100.00 |
100.00 |
| dsDNA - Iridoviridae |
| Manitoba iridescent virus 1* |
1 |
137 |
16.99 |
16.99 |
16.99 |
78.74 |
78.74 |
78.74 |
78.74 |
| dsRNA - Birnaviridae |
| Ballard Lake virus |
48 |
682,541 |
431.81 |
10.84 |
2,260.59 |
99.86 |
93.27 |
100.00 |
100.00 |
| dsRNA - Partitiviridae |
| Partitivirus-like Culex mosquito virus |
12 |
4,477 |
23.28 |
11.49 |
45.83 |
100.00 |
100.00 |
100.00 |
100.00 |
| dsRNA - Totiviridae |
| Gouley virus |
3 |
117 |
12.22 |
10.42 |
14.89 |
95.04 |
92.86 |
97.67 |
94.59 |
| Manitoba toti-like virus 1* |
5 |
13,869 |
39.82 |
11.18 |
127.46 |
69.92 |
67.43 |
73.11 |
68.10 |
| Manitoba toti-like virus 2* |
1 |
418 |
13.45 |
13.45 |
13.45 |
60.24 |
60.24 |
60.24 |
60.24 |
| Manitoba toti-like virus 3* |
4 |
1,772 |
15.11 |
11.20 |
18.40 |
73.53 |
72.22 |
74.90 |
73.50 |
| Manitoba toti-like virus 4* |
1 |
702 |
14.41 |
14.41 |
14.41 |
89.07 |
89.07 |
89.07 |
89.07 |
| Snelk virus |
4 |
496 |
41.15 |
23.15 |
67.61 |
100.00 |
100.00 |
100.00 |
100.00 |
| ssDNA - Parvoviridae |
| Aedes albopictus densovirus |
4 |
14,051 |
139.82 |
94.27 |
203.18 |
97.46 |
89.86 |
100.00 |
100.00 |
| Aedes vexans densovirus isolate |
1 |
594 |
17.69 |
17.69 |
17.69 |
100.00 |
100.00 |
100.00 |
100.00 |
| Culex densovirus |
8 |
6,035 |
127.22 |
11.65 |
490.03 |
99.56 |
97.71 |
100.00 |
100.00 |
| MAG: Grus japonensis parvoviridae sp |
1 |
366 |
60.82 |
60.82 |
60.82 |
100.00 |
100.00 |
100.00 |
100.00 |
Virus Detections by Species
virus_master_2023 %>%
group_by(mosquito_species, virus_name) %>%
summarise(count = n_distinct(virus_name, sample_number)) %>%
pivot_wider(names_from = mosquito_species, values_from = count, values_fill = 0) %>%
mutate("Aedes canadensis \n Total: 1" = (`Aedes canadensis` / 1) * 100,
"Aedes vexans \n Total: 19" = (`Aedes vexans` / 19) * 100,
"Anopheles earlei \n Total: 1" = (`Anopheles earlei` / 1) * 100,
"Coquillettidia perturbans \n Total: 6" = (`Coquillettidia perturbans` / 6) * 100,
"Culex tarsalis \n Total: 11" = (`Culex tarsalis` / 11) * 100,
"Ochlerotatus dorsalis \n Total: 5" = (`Ochlerotatus dorsalis` / 5) * 100,
"Ochlerotatus flavescens \n Total: 1" = (`Ochlerotatus flavescens` / 1) * 100,
"Ochlerotatus triseriatus \n Total: 1" = (`Ochlerotatus triseriatus` / 1) * 100,
) %>% select(-`Aedes canadensis`:-`Ochlerotatus triseriatus`) %>%
left_join(virus_lineage, by = "virus_name") %>%
left_join(years, by = "virus_name") %>%
relocate("2020", .after = "virus_name") %>% relocate("2021", .after = "2020") %>%
mutate(across(.cols = c("2020", "2021"),
~case_when(. == "1" ~ TRUE,
. == "0" ~ FALSE))) %>%
mutate(across(.cols = "virus_name",
~case_when(grepl("Manitoba", virus_name) & !grepl("Manitoba virus", virus_name) ~
paste0(., "*"),
TRUE ~ .))) %>%
group_by(genome, viral_family) %>%
arrange(genome, viral_family) %>%
gt() %>%
fmt_number(columns = "Aedes canadensis \n Total: 1":"Ochlerotatus triseriatus \n Total: 1",
decimals = 2) %>%
tab_spanner(label = "Percent of Libraries Detected by Species",
columns = c("Aedes canadensis \n Total: 1":"Ochlerotatus triseriatus \n Total: 1")) %>%
tab_spanner(label = html("Years Detected"),
columns = c("2020":"2021")) %>%
cols_label(
"Aedes canadensis \n Total: 1" = html("<em>Aedes canadensis</em> <br> Total: 1"),
"Aedes vexans \n Total: 19" = html("<em>Aedes vexans</em> <br> Total: 19"),
"Anopheles earlei \n Total: 1" = html("<em>Anopheles earlei</em> <br> Total: 1"),
"Coquillettidia perturbans \n Total: 6" = html("<em>Coquillettidia perturbans</em> <br> Total: 6"),
"Culex tarsalis \n Total: 11" = html("<em>Culex tarsalis</em> <br> Total: 11"),
"Ochlerotatus dorsalis \n Total: 5" = html("<em>Ochlerotatus dorsalis</em> <br> Total: 5"),
"Ochlerotatus flavescens \n Total: 1" = html("<em>Ochlerotatus flavescens</em> <br> Total: 1"),
"Ochlerotatus triseriatus \n Total: 1" = html("<em>Ochlerotatus triseriatus</em> <br> Total: 1"),
virus_name = "Virus") %>%
cols_align(align = "center",
columns = "2020":"Ochlerotatus triseriatus \n Total: 1") %>%
tab_style(
style = list(cell_fill(color = "grey"),
cell_text(weight = "bold")),
locations = cells_row_groups(groups = everything())) %>%
data_color(columns = `Aedes canadensis \n Total: 1`:last_col(),
palette = "viridis",
direction = "row") %>%
tab_style(
style = cell_borders(
sides = "left",
weight = px(2),
color = "black"),
locations = cells_body(
columns = c("Aedes canadensis \n Total: 1"))) %>%
tab_footnote("* Putatively novel virus") %>%
tab_options(., container.width = 1500)
| Virus |
Years Detected
|
Percent of Libraries Detected by Species
|
| 2020 |
2021 |
Aedes canadensis Total: 1 |
Aedes vexans Total: 19 |
Anopheles earlei Total: 1 |
Coquillettidia perturbans Total: 6 |
Culex tarsalis Total: 11 |
Ochlerotatus dorsalis Total: 5 |
Ochlerotatus flavescens Total: 1 |
Ochlerotatus triseriatus Total: 1 |
| +ssRNA - Dicistroviridae |
| Black queen cell virus |
TRUE |
FALSE |
0.00 |
5.26 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
| Soybean thrips dicistrovirus |
TRUE |
TRUE |
0.00 |
15.79 |
0.00 |
0.00 |
45.45 |
20.00 |
0.00 |
0.00 |
| Manitoba dicistro-like virus 1* |
TRUE |
FALSE |
0.00 |
0.00 |
0.00 |
0.00 |
9.09 |
0.00 |
0.00 |
0.00 |
| +ssRNA - Flaviviridae |
| Inari jingmenvirus |
TRUE |
FALSE |
0.00 |
5.26 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
| Placeda virus |
TRUE |
TRUE |
0.00 |
10.53 |
0.00 |
0.00 |
100.00 |
0.00 |
0.00 |
0.00 |
| +ssRNA - Iflaviridae |
| Hanko iflavirus 1 |
TRUE |
TRUE |
0.00 |
21.05 |
0.00 |
16.67 |
9.09 |
100.00 |
100.00 |
0.00 |
| Hanko iflavirus 2 |
TRUE |
TRUE |
0.00 |
78.95 |
0.00 |
16.67 |
0.00 |
40.00 |
0.00 |
0.00 |
| Hubei arthropod virus 1 |
FALSE |
TRUE |
0.00 |
5.26 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
| Manitoba iflavirus 1* |
TRUE |
TRUE |
0.00 |
36.84 |
0.00 |
33.33 |
27.27 |
80.00 |
100.00 |
0.00 |
| Manitoba picorna-like virus 1* |
TRUE |
TRUE |
0.00 |
100.00 |
0.00 |
0.00 |
0.00 |
60.00 |
0.00 |
0.00 |
| Cafluga virus |
TRUE |
FALSE |
0.00 |
0.00 |
0.00 |
16.67 |
0.00 |
20.00 |
0.00 |
0.00 |
| Soybean thrips iflavirus 4 |
TRUE |
FALSE |
0.00 |
0.00 |
0.00 |
16.67 |
0.00 |
0.00 |
0.00 |
0.00 |
| Culex Iflavi-like virus 4 |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
63.64 |
0.00 |
0.00 |
0.00 |
| Culex iflavilike virus 3 |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
100.00 |
0.00 |
0.00 |
0.00 |
| Yongsan picorna-like virus 2 |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
27.27 |
0.00 |
0.00 |
0.00 |
| +ssRNA - Luteoviridae |
| Marma virus |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
16.67 |
100.00 |
0.00 |
0.00 |
0.00 |
| +ssRNA - Narnaviridae |
| Manitoba narnavirus 1* |
FALSE |
TRUE |
0.00 |
0.00 |
100.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
| Culex narnavirus 1 |
TRUE |
FALSE |
0.00 |
0.00 |
0.00 |
0.00 |
9.09 |
0.00 |
0.00 |
0.00 |
| +ssRNA - Negevirus |
| Manitoba mononega-like virus 1* |
TRUE |
TRUE |
100.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
100.00 |
| Serbia mononega-like virus 1 |
FALSE |
TRUE |
100.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
| Cordoba virus |
FALSE |
TRUE |
0.00 |
15.79 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
| Manitoba mononega-like virus 2* |
TRUE |
TRUE |
0.00 |
15.79 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
| Mekrijarvi Negevirus |
TRUE |
TRUE |
0.00 |
15.79 |
0.00 |
0.00 |
0.00 |
60.00 |
0.00 |
0.00 |
| Utsjoki negevirus 3 |
TRUE |
FALSE |
0.00 |
10.53 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
| +ssRNA - Nodaviridae |
| Hubei noda-like virus 12 |
FALSE |
TRUE |
0.00 |
0.00 |
0.00 |
16.67 |
9.09 |
0.00 |
0.00 |
0.00 |
| +ssRNA - Rhabdoviridae |
| Manitoba Rhabdovirus 1* |
TRUE |
TRUE |
0.00 |
100.00 |
0.00 |
0.00 |
0.00 |
20.00 |
0.00 |
0.00 |
| +ssRNA - Tombusviridae |
| Des Moines River virus |
TRUE |
FALSE |
0.00 |
5.26 |
0.00 |
0.00 |
27.27 |
0.00 |
0.00 |
0.00 |
| Hubei mosquito virus 4 |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
54.55 |
0.00 |
0.00 |
0.00 |
| Manitoba tombus-like virus 1* |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
45.45 |
0.00 |
0.00 |
0.00 |
| +ssRNA - Tymoviridae |
| Hubei macula-like virus 3 |
TRUE |
TRUE |
0.00 |
21.05 |
0.00 |
0.00 |
0.00 |
20.00 |
0.00 |
0.00 |
| Manitoba tymo-like virus 1* |
FALSE |
TRUE |
0.00 |
5.26 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
| +ssRNA - Virgaviridae |
| Hubei virga-like virus 2 |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
36.36 |
0.00 |
0.00 |
0.00 |
| Manitoba virgavirus 1* |
TRUE |
FALSE |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
20.00 |
0.00 |
0.00 |
| -ssRNA - Chuviridae |
| Chuvirus |
TRUE |
TRUE |
0.00 |
89.47 |
0.00 |
0.00 |
9.09 |
60.00 |
0.00 |
0.00 |
| -ssRNA - Culex rhabdovirus |
| Culex rhabdovirus |
FALSE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
18.18 |
0.00 |
0.00 |
0.00 |
| -ssRNA - Flanders hapavirus |
| Flanders hapavirus |
TRUE |
TRUE |
0.00 |
15.79 |
0.00 |
0.00 |
90.91 |
0.00 |
0.00 |
0.00 |
| -ssRNA - Orthomyxoviridae |
| Wuhan mosquito virus 6 |
TRUE |
TRUE |
0.00 |
10.53 |
0.00 |
16.67 |
100.00 |
0.00 |
0.00 |
0.00 |
| Astopletus virus |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
100.00 |
0.00 |
0.00 |
0.00 |
| -ssRNA - Peribunyaviridae |
| Culex bunyavirus 2 |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
100.00 |
0.00 |
0.00 |
0.00 |
| -ssRNA - Rhabdoviridae |
| Canya virus |
TRUE |
TRUE |
100.00 |
0.00 |
0.00 |
0.00 |
90.91 |
0.00 |
0.00 |
0.00 |
| Manitoba rhabdovirus 2* |
TRUE |
FALSE |
0.00 |
5.26 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
| Culex Rhabdo-like virus |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
27.27 |
0.00 |
0.00 |
0.00 |
| Elisy virus |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
45.45 |
0.00 |
0.00 |
0.00 |
| Manitoba virus |
FALSE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
9.09 |
0.00 |
0.00 |
0.00 |
| Merida virus |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
100.00 |
0.00 |
0.00 |
0.00 |
| dsDNA - Iridoviridae |
| Manitoba iridescent virus 1* |
FALSE |
TRUE |
0.00 |
0.00 |
0.00 |
16.67 |
0.00 |
0.00 |
0.00 |
0.00 |
| dsRNA - Birnaviridae |
| Ballard Lake virus |
TRUE |
TRUE |
0.00 |
100.00 |
0.00 |
66.67 |
18.18 |
20.00 |
0.00 |
0.00 |
| dsRNA - Partitiviridae |
| Partitivirus-like Culex mosquito virus |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
100.00 |
0.00 |
0.00 |
0.00 |
| dsRNA - Totiviridae |
| Manitoba toti-like virus 2* |
FALSE |
TRUE |
100.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
| Manitoba toti-like virus 1* |
TRUE |
TRUE |
0.00 |
10.53 |
0.00 |
0.00 |
27.27 |
0.00 |
0.00 |
0.00 |
| Gouley virus |
TRUE |
FALSE |
0.00 |
0.00 |
0.00 |
0.00 |
27.27 |
0.00 |
0.00 |
0.00 |
| Snelk virus |
TRUE |
TRUE |
0.00 |
0.00 |
0.00 |
0.00 |
36.36 |
0.00 |
0.00 |
0.00 |
| Manitoba toti-like virus 3* |
TRUE |
FALSE |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
80.00 |
0.00 |
0.00 |
| Manitoba toti-like virus 4* |
TRUE |
FALSE |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
100.00 |
| ssDNA - Parvoviridae |
| Aedes vexans densovirus isolate |
FALSE |
TRUE |
0.00 |
5.26 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
0.00 |
| Culex densovirus |
TRUE |
TRUE |
0.00 |
5.26 |
0.00 |
66.67 |
9.09 |
0.00 |
0.00 |
0.00 |
| Aedes albopictus densovirus |
FALSE |
TRUE |
0.00 |
0.00 |
0.00 |
33.33 |
9.09 |
0.00 |
0.00 |
0.00 |
| MAG: Grus japonensis parvoviridae sp |
FALSE |
TRUE |
0.00 |
0.00 |
0.00 |
16.67 |
0.00 |
0.00 |
0.00 |
0.00 |
Virus Profiles
Below are summaries for each virus detected.
virus_profile_table <- function(virus) {
virus_table <<- virus_master_2023 %>%
filter(virus_name == virus) %>%
#select(-"contig_sequences") %>%
summarise(
num_species = n_distinct(mosquito_species),
num_contig = n(),
longest_contig = max(contig_length),
shortest_contig = min(contig_length),
avg_nt_id = mean(greatest_identity_percent),
max_nt_id = max(greatest_identity_percent),
min_nt_id = min(greatest_identity_percent)
) %>%
gt() %>%
cols_label(
longest_contig = "Max Contig",
shortest_contig = "Min Contig",
avg_nt_id = "Average aa Id",
max_nt_id = "Max aa Id",
min_nt_id = "Min aa Id",
num_contig = "n Contigs",
num_species = "n Species"
) %>%
tab_header(
title = paste0("Summary Stats for", " ", virus)
) %>%
fmt_number(
columns = 4:last_col(),
decimals = 2
)
}
# Arrange df for virus reporting
virus_master_2023 <<- virus_master_2023 %>%
mutate(across(.cols = "virus_name",
~case_when(grepl("Manitoba", virus_name) & !grepl("Manitoba virus", virus_name) ~
paste0(., "*"),
TRUE ~ .))) %>%
arrange(desc(genome)) %>%
drop_na(virus_name)
for(virus in unique(virus_master_2023$virus_name)) {
genome <- virus_master_2023 %>%
arrange(desc(genome)) %>%
drop_na(virus_name) %>%
filter(virus_name == virus) %>%
pull(genome) %>%
head(1)
genome <- paste0(genome)
family <- virus_master_2023 %>%
filter(virus_name == virus) %>%
pull(viral_family) %>%
head(1)
family <- paste0(family)
sample_detections <- virus_master_2023 %>%
filter(virus_name == virus) %>%
distinct(sample_number)
sample_detections <- paste0(nrow(sample_detections))
cat('\n\n#', virus, '\n\n')
cat('\n\n>', virus, 'has a ', genome, 'genome and is a member of the family', family, 'and was detected in', sample_detections, 'sample(s).', 'See summary figures and tables below.', '\n\n')
print(suppressMessages(
virus_master_2023 %>%
filter(virus_name == virus) %>%
select(-"contig_sequence") %>%
group_by(mosquito_species, collection_year, location_pool) %>%
summarise(n = n_distinct(sample_number)) %>%
ggplot(aes(x = mosquito_species, y = n, fill = location_pool)) +
geom_col() +
facet_grid(. ~ collection_year) +
scale_fill_viridis_d("Location", option = "magma") +
theme_bw() +
labs(title = paste0("Number of Virus Detections"),
subtitle = paste0("For ", virus, " by Year, Species and Location")) +
theme(plot.title = element_text(size = 16, face = "bold"),
axis.title.x = element_blank(),
axis.text.x = element_text(angle = 45, vjust = 0.5))
))
cat('\n\n###', 'Years {.unlisted .unnumbered}', '\n\n')
print(htmltools::tagList(
virus_master_2023 %>%
filter(virus_name == virus) %>%
group_by(collection_year) %>%
summarise(n = n_distinct(sample_number)) %>%
gt() %>%
cols_label(collection_year = "Year(s) Detected")
))
cat('\n\n###', 'Locations {.unlisted .unnumbered}', '\n\n')
print(htmltools::tagList(
virus_master_2023 %>%
filter(virus_name == virus) %>%
group_by(location_pool) %>%
summarise(n = n_distinct(sample_number)) %>%
gt() %>%
cols_label(location_pool = "Location(s) Detected")
))
cat('\n\n###', 'Mosquito Species {.unlisted .unnumbered}', '\n\n')
print(htmltools::tagList(
virus_master_2023 %>%
filter(virus_name == virus) %>%
group_by(mosquito_species) %>%
summarise(n = n_distinct(sample_number)) %>%
gt() %>%
cols_label(mosquito_species = "Mosquito Hosts")
))
cat('\n\n###', 'Contig Summary {.unlisted .unnumbered}', '\n\n')
virus_profile_table(virus)
print(htmltools::tagList(virus_table))
cat('\n\n###', 'aa Identity Summary {.unlisted .unnumbered}', '\n\n')
print(suppressMessages(
virus_master_2023 %>%
filter(virus_name == virus) %>%
select(-"contig_sequence") %>%
ggplot(aes(x = greatest_identity_percent)) +
geom_histogram(aes(fill = greatest_identity_percent < 90)) +
geom_vline(xintercept = 90, linetype = "dotted", colour = "firebrick") +
theme_bw() +
labs(title = paste0("aa Identity"),
subtitle = paste0("For ", virus),
x = "aa Identity",
y = "Number of Contigs") +
theme(plot.title = element_text(size = 16, face = "bold"),
axis.title.x = element_blank(),
axis.text.x = element_text(angle = 45, vjust = 0.5)) +
theme(legend.position = "none")
))
}
Culex densovirus
Culex densovirus has a ssDNA genome and is a member of the family
Parvoviridae and was detected in 6 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
3
|
|
2021
|
3
|
Locations
|
Location(s) Detected
|
n
|
|
Cypress River
|
3
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake
|
1
|
|
Western MB
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
1
|
|
Coquillettidia perturbans
|
4
|
|
Culex tarsalis
|
1
|
Contig Summary
|
Summary Stats for Culex densovirus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
3
|
8
|
1697
|
393.00
|
99.56
|
100.00
|
97.71
|
aa Identity Summary

Aedes albopictus densovirus
Aedes albopictus densovirus has a ssDNA genome and is a member of the
family Parvoviridae and was detected in 3 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2021
|
3
|
Locations
|
Location(s) Detected
|
n
|
|
Cypress River
|
2
|
|
Virden
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Coquillettidia perturbans
|
2
|
|
Culex tarsalis
|
1
|
Contig Summary
|
Summary Stats for Aedes albopictus densovirus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
4
|
3355
|
323.00
|
97.46
|
100.00
|
89.86
|
aa Identity Summary

Aedes vexans densovirus isolate
Aedes vexans densovirus isolate has a ssDNA genome and is a member of
the family Parvoviridae and was detected in 1 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2021
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Cypress River
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
1
|
Contig Summary
|
Summary Stats for Aedes vexans densovirus isolate
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
3367
|
3,367.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

MAG: Grus japonensis parvoviridae sp
MAG: Grus japonensis parvoviridae sp has a ssDNA genome and is a
member of the family Parvoviridae and was detected in 1 sample(s). See
summary figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2021
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Cypress River
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Coquillettidia perturbans
|
1
|
Contig Summary
|
Summary Stats for MAG: Grus japonensis parvoviridae sp
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
570
|
570.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Snelk virus
Snelk virus has a dsRNA genome and is a member of the family
Totiviridae and was detected in 4 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
3
|
|
2021
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
1
|
|
Carberry
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake, Virden & Souris
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
4
|
Contig Summary
|
Summary Stats for Snelk virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
4
|
295
|
262.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Partitivirus-like Culex mosquito virus
Partitivirus-like Culex mosquito virus has a dsRNA genome and is a
member of the family Partitiviridae and was detected in 11 sample(s).
See summary figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
5
|
|
2021
|
6
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
3
|
|
Carberry
|
1
|
|
Cypress River
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake
|
1
|
|
Shoal Lake, Virden & Souris
|
1
|
|
Souris
|
1
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
11
|
Contig Summary
|
Summary Stats for Partitivirus-like Culex mosquito virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
12
|
1751
|
299.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Gouley virus
Gouley virus has a dsRNA genome and is a member of the family
Totiviridae and was detected in 3 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
3
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake, Virden & Souris
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
3
|
Contig Summary
|
Summary Stats for Gouley virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
3
|
368
|
263.00
|
95.04
|
97.67
|
92.86
|
aa Identity Summary

Manitoba toti-like virus 1*
Manitoba toti-like virus 1* has a dsRNA genome and is a member of the
family Totiviridae and was detected in 5 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
2
|
|
2021
|
3
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
1
|
|
Cypress River
|
1
|
|
Shoal Lake
|
3
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
2
|
|
Culex tarsalis
|
3
|
Contig Summary
|
Summary Stats for Manitoba toti-like virus 1*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
5
|
8930
|
2,012.00
|
69.92
|
73.11
|
67.43
|
aa Identity Summary

Ballard Lake virus
Ballard Lake virus has a dsRNA genome and is a member of the family
Birnaviridae and was detected in 26 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
10
|
|
2021
|
16
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
2
|
|
Brandon
|
4
|
|
Carberry
|
1
|
|
Cypress River
|
3
|
|
Cypress River & Carberry
|
1
|
|
Killarney
|
2
|
|
Newdale
|
1
|
|
Shoal Lake
|
5
|
|
Souris
|
2
|
|
Virden
|
2
|
|
Virden & Souris
|
1
|
|
WPG Insect Contol
|
2
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
19
|
|
Coquillettidia perturbans
|
4
|
|
Culex tarsalis
|
2
|
|
Ochlerotatus dorsalis
|
1
|
Contig Summary
|
Summary Stats for Ballard Lake virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
4
|
48
|
3472
|
707.00
|
99.86
|
100.00
|
93.27
|
aa Identity Summary

Manitoba toti-like virus 2*
Manitoba toti-like virus 2* has a dsRNA genome and is a member of the
family Totiviridae and was detected in 1 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2021
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes canadensis
|
1
|
Contig Summary
|
Summary Stats for Manitoba toti-like virus 2*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
3123
|
3,123.00
|
60.24
|
60.24
|
60.24
|
aa Identity Summary

Manitoba toti-like virus 4*
Manitoba toti-like virus 4* has a dsRNA genome and is a member of the
family Totiviridae and was detected in 1 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Multiple
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Ochlerotatus triseriatus
|
1
|
Contig Summary
|
Summary Stats for Manitoba toti-like virus 4*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
4909
|
4,909.00
|
89.07
|
89.07
|
89.07
|
aa Identity Summary

Manitoba toti-like virus 3*
Manitoba toti-like virus 3* has a dsRNA genome and is a member of the
family Totiviridae and was detected in 4 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
4
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain & Killarney
|
1
|
|
Brandon
|
1
|
|
Shoal Lake
|
1
|
|
Virden & Souris
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Ochlerotatus dorsalis
|
4
|
Contig Summary
|
Summary Stats for Manitoba toti-like virus 3*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
4
|
4626
|
1,821.00
|
73.53
|
74.90
|
72.22
|
aa Identity Summary

Manitoba iridescent virus 1*
Manitoba iridescent virus 1* has a dsDNA genome and is a member of
the family Iridoviridae and was detected in 1 sample(s). See summary
figures and tables below.

Years
Locations
|
Location(s) Detected
|
n
|
|
Cypress River
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Coquillettidia perturbans
|
1
|
Contig Summary
|
Summary Stats for Manitoba iridescent virus 1*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
797
|
797.00
|
78.74
|
78.74
|
78.74
|
aa Identity Summary

Chuvirus
Chuvirus has a -ssRNA genome and is a member of the family Chuviridae
and was detected in 21 sample(s). See summary figures and tables
below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
9
|
|
2021
|
12
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
2
|
|
Boissevain & Killarney
|
1
|
|
Brandon
|
3
|
|
Carberry
|
2
|
|
Cypress River
|
1
|
|
Cypress River & Carberry
|
1
|
|
Killarney
|
1
|
|
Newdale
|
1
|
|
Shoal Lake
|
6
|
|
Souris
|
1
|
|
Virden
|
1
|
|
Virden & Souris
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
17
|
|
Culex tarsalis
|
1
|
|
Ochlerotatus dorsalis
|
3
|
Contig Summary
|
Summary Stats for Chuvirus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
3
|
39
|
6799
|
433.00
|
97.49
|
100.00
|
89.66
|
aa Identity Summary

Merida virus
Merida virus has a -ssRNA genome and is a member of the family
Rhabdoviridae and was detected in 11 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
5
|
|
2021
|
6
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
3
|
|
Carberry
|
1
|
|
Cypress River
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake
|
1
|
|
Shoal Lake, Virden & Souris
|
1
|
|
Souris
|
1
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
11
|
Contig Summary
|
Summary Stats for Merida virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
55
|
7483
|
262.00
|
99.86
|
100.00
|
97.14
|
aa Identity Summary

Wuhan mosquito virus 6
Wuhan mosquito virus 6 has a -ssRNA genome and is a member of the
family Orthomyxoviridae and was detected in 14 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
7
|
|
2021
|
7
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
4
|
|
Carberry
|
1
|
|
Cypress River
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake
|
2
|
|
Shoal Lake, Virden & Souris
|
1
|
|
Souris
|
1
|
|
WPG Insect Contol
|
1
|
|
Western MB
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
2
|
|
Coquillettidia perturbans
|
1
|
|
Culex tarsalis
|
11
|
Contig Summary
|
Summary Stats for Wuhan mosquito virus 6
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
3
|
101
|
2467
|
252.00
|
99.76
|
100.00
|
97.69
|
aa Identity Summary

Canya virus
Canya virus has a -ssRNA genome and is a member of the family
Rhabdoviridae and was detected in 11 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
4
|
|
2021
|
7
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
4
|
|
Carberry
|
1
|
|
Cypress River
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake
|
1
|
|
Souris
|
1
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes canadensis
|
1
|
|
Culex tarsalis
|
10
|
Contig Summary
|
Summary Stats for Canya virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
14
|
6071
|
522.00
|
87.32
|
100.00
|
66.04
|
aa Identity Summary

Flanders hapavirus
Flanders hapavirus has a -ssRNA genome and is a member of the family
Flanders hapavirus and was detected in 13 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
5
|
|
2021
|
8
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
3
|
|
Carberry
|
1
|
|
Cypress River
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake
|
3
|
|
Shoal Lake, Virden & Souris
|
1
|
|
Souris
|
1
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
3
|
|
Culex tarsalis
|
10
|
Contig Summary
|
Summary Stats for Flanders hapavirus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
81
|
8773
|
263.00
|
99.90
|
100.00
|
95.96
|
aa Identity Summary

Astopletus virus
Astopletus virus has a -ssRNA genome and is a member of the family
Orthomyxoviridae and was detected in 11 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
5
|
|
2021
|
6
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
3
|
|
Carberry
|
1
|
|
Cypress River
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake
|
1
|
|
Shoal Lake, Virden & Souris
|
1
|
|
Souris
|
1
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
11
|
Contig Summary
|
Summary Stats for Astopletus virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
29
|
1044
|
464.00
|
99.06
|
100.00
|
95.77
|
aa Identity Summary

Elisy virus
Elisy virus has a -ssRNA genome and is a member of the family
Rhabdoviridae and was detected in 5 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
3
|
|
2021
|
2
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
3
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
5
|
Contig Summary
|
Summary Stats for Elisy virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
21
|
1817
|
373.00
|
99.27
|
100.00
|
96.41
|
aa Identity Summary

Culex Rhabdo-like virus
Culex Rhabdo-like virus has a -ssRNA genome and is a member of the
family Rhabdoviridae and was detected in 3 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
1
|
|
2021
|
2
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
3
|
Contig Summary
|
Summary Stats for Culex Rhabdo-like virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
4
|
1973
|
672.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Culex bunyavirus 2
Culex bunyavirus 2 has a -ssRNA genome and is a member of the family
Peribunyaviridae and was detected in 11 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
5
|
|
2021
|
6
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
3
|
|
Carberry
|
1
|
|
Cypress River
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake
|
1
|
|
Shoal Lake, Virden & Souris
|
1
|
|
Souris
|
1
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
11
|
Contig Summary
|
Summary Stats for Culex bunyavirus 2
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
19
|
1896
|
350.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Manitoba virus
Manitoba virus has a -ssRNA genome and is a member of the family
Rhabdoviridae and was detected in 1 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2021
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Shoal Lake
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
1
|
Contig Summary
|
Summary Stats for Manitoba virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
5
|
1589
|
500.00
|
99.76
|
100.00
|
98.80
|
aa Identity Summary

Culex rhabdovirus
Culex rhabdovirus has a -ssRNA genome and is a member of the family
Culex rhabdovirus and was detected in 2 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2021
|
2
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
1
|
|
Cypress River
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
2
|
Contig Summary
|
Summary Stats for Culex rhabdovirus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
2
|
429
|
359.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Manitoba rhabdovirus 2*
Manitoba rhabdovirus 2* has a -ssRNA genome and is a member of the
family Rhabdoviridae and was detected in 1 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Shoal Lake
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
1
|
Contig Summary
|
Summary Stats for Manitoba rhabdovirus 2*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
4648
|
4,648.00
|
75.00
|
75.00
|
75.00
|
aa Identity Summary

Manitoba picorna-like virus 1*
Manitoba picorna-like virus 1* has a +ssRNA genome and is a member of
the family Iflaviridae and was detected in 22 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
11
|
|
2021
|
11
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
2
|
|
Boissevain & Killarney
|
1
|
|
Brandon
|
4
|
|
Carberry
|
1
|
|
Cypress River
|
1
|
|
Cypress River & Carberry
|
1
|
|
Killarney
|
2
|
|
Newdale
|
1
|
|
Shoal Lake
|
5
|
|
Souris
|
1
|
|
Virden
|
1
|
|
Virden & Souris
|
1
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
19
|
|
Ochlerotatus dorsalis
|
3
|
Contig Summary
|
Summary Stats for Manitoba picorna-like virus 1*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
86
|
4085
|
510.00
|
87.73
|
100.00
|
73.93
|
aa Identity Summary

Manitoba Rhabdovirus 1*
Manitoba Rhabdovirus 1* has a +ssRNA genome and is a member of the
family Rhabdoviridae and was detected in 20 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
9
|
|
2021
|
11
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
2
|
|
Brandon
|
3
|
|
Carberry
|
1
|
|
Cypress River
|
1
|
|
Cypress River & Carberry
|
1
|
|
Killarney
|
2
|
|
Newdale
|
1
|
|
Shoal Lake
|
5
|
|
Souris
|
1
|
|
Virden
|
1
|
|
Virden & Souris
|
1
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
19
|
|
Ochlerotatus dorsalis
|
1
|
Contig Summary
|
Summary Stats for Manitoba Rhabdovirus 1*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
37
|
8033
|
718.00
|
83.22
|
93.01
|
69.42
|
aa Identity Summary

Hanko iflavirus 1
Hanko iflavirus 1 has a +ssRNA genome and is a member of the family
Iflaviridae and was detected in 12 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
8
|
|
2021
|
4
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain & Killarney
|
1
|
|
Brandon
|
1
|
|
Cypress River
|
1
|
|
Newdale
|
1
|
|
Shoal Lake
|
4
|
|
Virden & Souris
|
1
|
|
WPG Insect Contol
|
3
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
4
|
|
Coquillettidia perturbans
|
1
|
|
Culex tarsalis
|
1
|
|
Ochlerotatus dorsalis
|
5
|
|
Ochlerotatus flavescens
|
1
|
Contig Summary
|
Summary Stats for Hanko iflavirus 1
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
5
|
30
|
9246
|
308.00
|
97.49
|
100.00
|
93.45
|
aa Identity Summary

Manitoba iflavirus 1*
Manitoba iflavirus 1* has a +ssRNA genome and is a member of the
family Iflaviridae and was detected in 17 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
16
|
|
2021
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain & Killarney
|
1
|
|
Brandon
|
5
|
|
Cypress River & Carberry
|
2
|
|
Killarney
|
1
|
|
Shoal Lake
|
4
|
|
Virden & Souris
|
1
|
|
WPG Insect Contol
|
2
|
|
Western MB
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
7
|
|
Coquillettidia perturbans
|
2
|
|
Culex tarsalis
|
3
|
|
Ochlerotatus dorsalis
|
4
|
|
Ochlerotatus flavescens
|
1
|
Contig Summary
|
Summary Stats for Manitoba iflavirus 1*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
5
|
25
|
9899
|
286.00
|
93.47
|
100.00
|
89.14
|
aa Identity Summary

Marma virus
Marma virus has a +ssRNA genome and is a member of the family
Luteoviridae and was detected in 12 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
6
|
|
2021
|
6
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
3
|
|
Carberry
|
1
|
|
Cypress River
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake
|
1
|
|
Shoal Lake, Virden & Souris
|
1
|
|
Souris
|
1
|
|
WPG Insect Contol
|
1
|
|
Western MB
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Coquillettidia perturbans
|
1
|
|
Culex tarsalis
|
11
|
Contig Summary
|
Summary Stats for Marma virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
23
|
3160
|
1,574.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Culex iflavilike virus 3
Culex iflavilike virus 3 has a +ssRNA genome and is a member of the
family Iflaviridae and was detected in 11 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
5
|
|
2021
|
6
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
3
|
|
Carberry
|
1
|
|
Cypress River
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake
|
1
|
|
Shoal Lake, Virden & Souris
|
1
|
|
Souris
|
1
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
11
|
Contig Summary
|
Summary Stats for Culex iflavilike virus 3
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
183
|
1824
|
250.00
|
99.49
|
100.00
|
94.19
|
aa Identity Summary

Placeda virus
Placeda virus has a +ssRNA genome and is a member of the family
Flaviviridae and was detected in 13 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
6
|
|
2021
|
7
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
4
|
|
Carberry
|
1
|
|
Cypress River
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake
|
2
|
|
Shoal Lake, Virden & Souris
|
1
|
|
Souris
|
1
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
2
|
|
Culex tarsalis
|
11
|
Contig Summary
|
Summary Stats for Placeda virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
169
|
11737
|
252.00
|
97.87
|
100.00
|
86.24
|
aa Identity Summary

Culex Iflavi-like virus 4
Culex Iflavi-like virus 4 has a +ssRNA genome and is a member of the
family Iflaviridae and was detected in 7 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
4
|
|
2021
|
3
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
3
|
|
Carberry
|
1
|
|
Cypress River & Carberry
|
1
|
|
Shoal Lake, Virden & Souris
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
7
|
Contig Summary
|
Summary Stats for Culex Iflavi-like virus 4
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
23
|
9811
|
285.00
|
99.81
|
100.00
|
96.20
|
aa Identity Summary

Hubei mosquito virus 4
Hubei mosquito virus 4 has a +ssRNA genome and is a member of the
family Tombusviridae and was detected in 6 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
3
|
|
2021
|
3
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
3
|
|
Carberry
|
1
|
|
Cypress River & Carberry
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
6
|
Contig Summary
|
Summary Stats for Hubei mosquito virus 4
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
6
|
5103
|
367.00
|
96.50
|
100.00
|
92.65
|
aa Identity Summary

Hubei virga-like virus 2
Hubei virga-like virus 2 has a +ssRNA genome and is a member of the
family Virgaviridae and was detected in 4 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
3
|
|
2021
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
2
|
|
Cypress River & Carberry
|
1
|
|
Souris
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
4
|
Contig Summary
|
Summary Stats for Hubei virga-like virus 2
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
6
|
947
|
290.00
|
99.04
|
100.00
|
96.27
|
aa Identity Summary

Des Moines River virus
Des Moines River virus has a +ssRNA genome and is a member of the
family Tombusviridae and was detected in 4 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
4
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
3
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
1
|
|
Culex tarsalis
|
3
|
Contig Summary
|
Summary Stats for Des Moines River virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
4
|
2361
|
1,070.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Manitoba dicistro-like virus 1*
Manitoba dicistro-like virus 1* has a +ssRNA genome and is a member
of the family Dicistroviridae and was detected in 1 sample(s). See
summary figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
1
|
Contig Summary
|
Summary Stats for Manitoba dicistro-like virus 1*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
7146
|
7,146.00
|
82.61
|
82.61
|
82.61
|
aa Identity Summary

Soybean thrips dicistrovirus
Soybean thrips dicistrovirus has a +ssRNA genome and is a member of
the family Dicistroviridae and was detected in 9 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
3
|
|
2021
|
6
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
2
|
|
Brandon
|
1
|
|
Carberry
|
1
|
|
Newdale
|
1
|
|
Shoal Lake
|
2
|
|
Souris
|
1
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
3
|
|
Culex tarsalis
|
5
|
|
Ochlerotatus dorsalis
|
1
|
Contig Summary
|
Summary Stats for Soybean thrips dicistrovirus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
3
|
11
|
9121
|
893.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Yongsan picorna-like virus 2
Yongsan picorna-like virus 2 has a +ssRNA genome and is a member of
the family Iflaviridae and was detected in 3 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
1
|
|
2021
|
2
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
2
|
|
Shoal Lake
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
3
|
Contig Summary
|
Summary Stats for Yongsan picorna-like virus 2
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
4
|
9573
|
2,367.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Manitoba tombus-like virus 1*
Manitoba tombus-like virus 1* has a +ssRNA genome and is a member of
the family Tombusviridae and was detected in 5 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
3
|
|
2021
|
2
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
2
|
|
Shoal Lake
|
1
|
|
Souris
|
1
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
5
|
Contig Summary
|
Summary Stats for Manitoba tombus-like virus 1*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
6
|
2351
|
1,687.00
|
79.73
|
100.00
|
72.73
|
aa Identity Summary

Culex narnavirus 1
Culex narnavirus 1 has a +ssRNA genome and is a member of the family
Narnaviridae and was detected in 1 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Culex tarsalis
|
1
|
Contig Summary
|
Summary Stats for Culex narnavirus 1
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
513
|
513.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Hubei noda-like virus 12
Hubei noda-like virus 12 has a +ssRNA genome and is a member of the
family Nodaviridae and was detected in 2 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2021
|
2
|
Locations
|
Location(s) Detected
|
n
|
|
Carberry
|
1
|
|
Virden
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Coquillettidia perturbans
|
1
|
|
Culex tarsalis
|
1
|
Contig Summary
|
Summary Stats for Hubei noda-like virus 12
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
2
|
4762
|
841.00
|
95.83
|
100.00
|
91.67
|
aa Identity Summary

Manitoba mononega-like virus 1*
Manitoba mononega-like virus 1* has a +ssRNA genome and is a member
of the family Negevirus and was detected in 2 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
1
|
|
2021
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
1
|
|
Multiple
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes canadensis
|
1
|
|
Ochlerotatus triseriatus
|
1
|
Contig Summary
|
Summary Stats for Manitoba mononega-like virus 1*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
3
|
11375
|
5,456.00
|
80.60
|
90.00
|
73.21
|
aa Identity Summary

Serbia mononega-like virus 1
Serbia mononega-like virus 1 has a +ssRNA genome and is a member of
the family Negevirus and was detected in 1 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2021
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes canadensis
|
1
|
Contig Summary
|
Summary Stats for Serbia mononega-like virus 1
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
2477
|
2,477.00
|
75.00
|
75.00
|
75.00
|
aa Identity Summary

Hubei macula-like virus 3
Hubei macula-like virus 3 has a +ssRNA genome and is a member of the
family Tymoviridae and was detected in 5 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
4
|
|
2021
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
2
|
|
Shoal Lake
|
2
|
|
Virden & Souris
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
4
|
|
Ochlerotatus dorsalis
|
1
|
Contig Summary
|
Summary Stats for Hubei macula-like virus 3
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
8
|
6059
|
2,954.00
|
96.13
|
100.00
|
92.15
|
aa Identity Summary

Hanko iflavirus 2
Hanko iflavirus 2 has a +ssRNA genome and is a member of the family
Iflaviridae and was detected in 18 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
11
|
|
2021
|
7
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
3
|
|
Cypress River & Carberry
|
1
|
|
Killarney
|
2
|
|
Newdale
|
1
|
|
Shoal Lake
|
5
|
|
Souris
|
1
|
|
Virden
|
1
|
|
Virden & Souris
|
1
|
|
WPG Insect Contol
|
2
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
15
|
|
Coquillettidia perturbans
|
1
|
|
Ochlerotatus dorsalis
|
2
|
Contig Summary
|
Summary Stats for Hanko iflavirus 2
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
3
|
29
|
9206
|
461.00
|
88.70
|
100.00
|
67.08
|
aa Identity Summary

Black queen cell virus
Black queen cell virus has a +ssRNA genome and is a member of the
family Dicistroviridae and was detected in 1 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Brandon
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
1
|
Contig Summary
|
Summary Stats for Black queen cell virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
2
|
6035
|
2,087.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Cordoba virus
Cordoba virus has a +ssRNA genome and is a member of the family
Negevirus and was detected in 3 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2021
|
3
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Cypress River
|
1
|
|
Souris
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
3
|
Contig Summary
|
Summary Stats for Cordoba virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
14
|
5133
|
610.00
|
97.10
|
100.00
|
93.75
|
aa Identity Summary

Mekrijarvi Negevirus
Mekrijarvi Negevirus has a +ssRNA genome and is a member of the
family Negevirus and was detected in 6 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
2
|
|
2021
|
4
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Boissevain & Killarney
|
1
|
|
Cypress River
|
1
|
|
Shoal Lake
|
1
|
|
Souris
|
1
|
|
Virden & Souris
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
3
|
|
Ochlerotatus dorsalis
|
3
|
Contig Summary
|
Summary Stats for Mekrijarvi Negevirus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
6
|
9873
|
3,125.00
|
94.13
|
100.00
|
90.78
|
aa Identity Summary

Manitoba mononega-like virus 2*
Manitoba mononega-like virus 2* has a +ssRNA genome and is a member
of the family Negevirus and was detected in 3 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
1
|
|
2021
|
2
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
|
Brandon
|
1
|
|
Souris
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
3
|
Contig Summary
|
Summary Stats for Manitoba mononega-like virus 2*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
3
|
9570
|
2,735.00
|
98.77
|
100.00
|
96.30
|
aa Identity Summary

Hubei arthropod virus 1
Hubei arthropod virus 1 has a +ssRNA genome and is a member of the
family Iflaviridae and was detected in 1 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2021
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Shoal Lake
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
1
|
Contig Summary
|
Summary Stats for Hubei arthropod virus 1
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
2967
|
2,967.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Soybean thrips iflavirus 4
Soybean thrips iflavirus 4 has a +ssRNA genome and is a member of the
family Iflaviridae and was detected in 1 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Coquillettidia perturbans
|
1
|
Contig Summary
|
Summary Stats for Soybean thrips iflavirus 4
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
3835
|
3,835.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Cafluga virus
Cafluga virus has a +ssRNA genome and is a member of the family
Iflaviridae and was detected in 2 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
2
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain & Killarney
|
1
|
|
WPG Insect Contol
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Coquillettidia perturbans
|
1
|
|
Ochlerotatus dorsalis
|
1
|
Contig Summary
|
Summary Stats for Cafluga virus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
2
|
2
|
3426
|
1,395.00
|
99.77
|
100.00
|
99.55
|
aa Identity Summary

Manitoba tymo-like virus 1*
Manitoba tymo-like virus 1* has a +ssRNA genome and is a member of
the family Tymoviridae and was detected in 1 sample(s). See summary
figures and tables below.

Years
|
Year(s) Detected
|
n
|
|
2021
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Boissevain
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
1
|
Contig Summary
|
Summary Stats for Manitoba tymo-like virus 1*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
6302
|
6,302.00
|
66.79
|
66.79
|
66.79
|
aa Identity Summary

Utsjoki negevirus 3
Utsjoki negevirus 3 has a +ssRNA genome and is a member of the family
Negevirus and was detected in 2 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
2
|
Locations
|
Location(s) Detected
|
n
|
|
Shoal Lake
|
1
|
|
Virden & Souris
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
2
|
Contig Summary
|
Summary Stats for Utsjoki negevirus 3
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
2
|
989
|
972.00
|
99.02
|
99.03
|
99.01
|
aa Identity Summary

Inari jingmenvirus
Inari jingmenvirus has a +ssRNA genome and is a member of the family
Flaviviridae and was detected in 1 sample(s). See summary figures and
tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Shoal Lake
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Aedes vexans
|
1
|
Contig Summary
|
Summary Stats for Inari jingmenvirus
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
1267
|
1,267.00
|
100.00
|
100.00
|
100.00
|
aa Identity Summary

Manitoba virgavirus 1*
Manitoba virgavirus 1* has a +ssRNA genome and is a member of the
family Virgaviridae and was detected in 1 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2020
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Shoal Lake
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Ochlerotatus dorsalis
|
1
|
Contig Summary
|
Summary Stats for Manitoba virgavirus 1*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
1
|
1675
|
1,675.00
|
83.83
|
83.83
|
83.83
|
aa Identity Summary

Manitoba narnavirus 1*
Manitoba narnavirus 1* has a +ssRNA genome and is a member of the
family Narnaviridae and was detected in 1 sample(s). See summary figures
and tables below.

Years
|
Year(s) Detected
|
n
|
|
2021
|
1
|
Locations
|
Location(s) Detected
|
n
|
|
Shoal Lake
|
1
|
Mosquito Species
|
Mosquito Hosts
|
n
|
|
Anopheles earlei
|
1
|
Contig Summary
|
Summary Stats for Manitoba narnavirus 1*
|
|
n Species
|
n Contigs
|
Max Contig
|
Min Contig
|
Average aa Id
|
Max aa Id
|
Min aa Id
|
|
1
|
2
|
1760
|
654.00
|
80.59
|
80.89
|
80.29
|
aa Identity Summary

---
title: "Mosquito Metagenomics Summary"
author: "Cole Baril"
date: "`r Sys.Date()`"
output: 
  html_document:
    toc: true
    toc_float: true
    theme: cosmo
    code_download: true
    code_folding: show
---

```{css toc-content, echo = FALSE}
#TOC {
  left: 25px;
  margin: 25px 0px 25px 0px;
}

.main-container {
    margin-left: 50px;
}
```

```{r setup, include=FALSE}
knitr::opts_chunk$set(echo = TRUE, message = FALSE, warnings = FALSE)
```

```{=html}
<style>
   table {
     display: block;
     overflow: auto;
   }

   blockquote {
   background-color: #33638dff;
   color:white;
  }

 </style>
```


# Libraries

```{r, load_libraries, message = FALSE, warning = FALSE}
require(pacman)
pacman::p_load(tidyverse, janitor, here, DT, gt, phylotools, assertr, readxl, patchwork, reshape2, ggtext, openxlsx, grid, tiff)
```

# Load Data

```{r, load_data, message = FALSE, warning = FALSE}
virus_master_2023 <- read_csv(here("Data/tblastx_master.csv"))
```

# BLAST Summary

```{r, read_contig_summary, message = FALSE, warning = FALSE}
library_summary <- virus_master_2023 %>%
  group_by(mosquito_species, sample_number) %>%
  summarise(n = n_distinct(mosquito_species)) %>%
  group_by(mosquito_species) %>%
  summarise(n = n()) %>%
  adorn_totals()

years <- virus_master_2023 %>% 
  group_by(collection_year, virus_name) %>% 
  summarise(count = n_distinct(virus_name, collection_year)) %>% 
  pivot_wider(names_from = collection_year, values_from = count, values_fill = 0)

virus_lineage <- virus_master_2023 %>% 
  group_by(virus_name, viral_family, genome) %>% 
  summarise(n = n_distinct(virus_name, viral_family, genome)) %>% 
  select(-"n")

virus_master_2023 %>% 
  group_by(virus_name) %>% 
  summarise(n_contigs = n(),
            mean_cov = mean(coverage),
            min_cov = min(coverage),
            max_cov = max(coverage),
            mean_pid = mean(greatest_identity_percent),
            med_pid = median(greatest_identity_percent),
            min_pid = min(greatest_identity_percent),
            max_pid = max(greatest_identity_percent),
            total_reads = sum(total_read_count)) %>% 
  left_join(virus_lineage, by = "virus_name") %>% 
  group_by(genome, viral_family) %>% 
  arrange(genome, viral_family) %>% 
  relocate(total_reads, .after = "n_contigs") %>% 
  mutate(across(.cols = "virus_name",
                ~case_when(grepl("Manitoba", virus_name) & !grepl("Manitoba virus", virus_name) ~ 
                             paste0(., "*"),
                           TRUE ~ .))) %>% 
  gt() %>% 
  fmt_number(columns = mean_cov:max_pid,
             decimals = 2) %>% 
  fmt_number(columns = total_reads,
             sep_mark = ",",
             decimals = 0) %>% 
  tab_spanner(label = "Coverage Depth", columns = c(mean_cov, min_cov, max_cov)) %>% 
  tab_spanner(label = "aa Percent Identity", columns = c(mean_pid, min_pid, max_pid, med_pid)) %>% 
  cols_label(
    n_contigs = "Contigs",
    total_reads = "Reads",
    mean_cov = "Mean", min_cov = "Min", max_cov = "Max",
    mean_pid = "Mean", min_pid = "Min", max_pid = "Max", med_pid = "Median",
    virus_name = "Virus") %>% 
  tab_style(
    style = list(cell_fill(color = "grey"),
                 cell_text(weight = "bold")),
    locations = cells_row_groups(groups = everything())
  ) %>% 
  tab_style(
    style = cell_borders(
      sides = "left",
      weight = px(2),
      color = "grey"),
    locations = cells_body(
      columns = c(mean_cov, mean_pid, "n_contigs")
    )
  ) %>% 
  data_color(
    columns = mean_pid:max_pid,
    palette = "viridis"
    
  ) %>% 
  tab_footnote("* Putatively novel virus.")
```

# Virus Detections by Species

```{r}
virus_master_2023 %>% 
  group_by(mosquito_species, virus_name) %>% 
  summarise(count = n_distinct(virus_name, sample_number)) %>% 
  pivot_wider(names_from = mosquito_species, values_from = count, values_fill = 0) %>% 
  mutate("Aedes canadensis \n Total: 1" = (`Aedes canadensis` / 1) * 100,
         "Aedes vexans \n Total: 19" = (`Aedes vexans` / 19) * 100,
         "Anopheles earlei \n Total: 1" = (`Anopheles earlei` / 1) * 100,
         "Coquillettidia perturbans \n Total: 6" = (`Coquillettidia perturbans` / 6) * 100,
         "Culex tarsalis \n Total: 11" = (`Culex tarsalis` / 11) * 100,
         "Ochlerotatus dorsalis \n Total: 5" = (`Ochlerotatus dorsalis` / 5) * 100,
         "Ochlerotatus flavescens \n Total: 1" = (`Ochlerotatus flavescens` / 1) * 100,
         "Ochlerotatus triseriatus \n Total: 1" = (`Ochlerotatus triseriatus` / 1) * 100,
  ) %>% select(-`Aedes canadensis`:-`Ochlerotatus triseriatus`) %>% 
  left_join(virus_lineage, by = "virus_name") %>% 
   left_join(years, by = "virus_name") %>% 
   relocate("2020", .after = "virus_name") %>% relocate("2021", .after = "2020") %>% 
   mutate(across(.cols = c("2020", "2021"),
                 ~case_when(. == "1" ~ TRUE,
                            . == "0" ~ FALSE))) %>% 
   mutate(across(.cols = "virus_name",
                 ~case_when(grepl("Manitoba", virus_name) & !grepl("Manitoba virus", virus_name) ~ 
                              paste0(., "*"),
                            TRUE ~ .))) %>% 
  group_by(genome, viral_family) %>% 
  arrange(genome, viral_family) %>% 
  gt() %>% 
  fmt_number(columns = "Aedes canadensis \n Total: 1":"Ochlerotatus triseriatus \n Total: 1",
             decimals = 2) %>% 
  tab_spanner(label = "Percent of Libraries Detected by Species", 
              columns = c("Aedes canadensis \n Total: 1":"Ochlerotatus triseriatus \n Total: 1")) %>% 
   tab_spanner(label = html("Years Detected"),
               columns = c("2020":"2021")) %>% 
  cols_label(
    "Aedes canadensis \n Total: 1" = html("<em>Aedes canadensis</em> <br> Total: 1"),
    "Aedes vexans \n Total: 19" = html("<em>Aedes vexans</em> <br> Total: 19"),
    "Anopheles earlei \n Total: 1" = html("<em>Anopheles earlei</em> <br> Total: 1"),
    "Coquillettidia perturbans \n Total: 6" = html("<em>Coquillettidia perturbans</em> <br> Total: 6"),
    "Culex tarsalis \n Total: 11" = html("<em>Culex tarsalis</em> <br> Total: 11"),
    "Ochlerotatus dorsalis \n Total: 5" = html("<em>Ochlerotatus dorsalis</em> <br> Total: 5"),
    "Ochlerotatus flavescens \n Total: 1" = html("<em>Ochlerotatus flavescens</em> <br> Total: 1"),
    "Ochlerotatus triseriatus \n Total: 1" = html("<em>Ochlerotatus triseriatus</em> <br> Total: 1"),
    virus_name = "Virus") %>% 
   cols_align(align = "center",
              columns = "2020":"Ochlerotatus triseriatus \n Total: 1") %>% 
   tab_style(
     style = list(cell_fill(color = "grey"),
                  cell_text(weight = "bold")),
     locations = cells_row_groups(groups = everything())) %>% 
   data_color(columns = `Aedes canadensis \n Total: 1`:last_col(),
              palette = "viridis", 
              direction = "row") %>% 
   tab_style(
     style = cell_borders(
       sides = "left",
       weight = px(2),
       color = "black"),
     locations = cells_body(
       columns = c("Aedes canadensis \n Total: 1"))) %>% 
  tab_footnote("* Putatively novel virus") %>% 
  tab_options(., container.width = 1500)
```

# Virus Profiles

> Below are summaries for each virus detected. 

```{r, virus_table}
virus_profile_table <- function(virus) {

 virus_table <<-  virus_master_2023 %>% 
  filter(virus_name == virus) %>% 
  #select(-"contig_sequences") %>% 
  summarise(
    num_species = n_distinct(mosquito_species),
    num_contig = n(),
    longest_contig = max(contig_length),
    shortest_contig = min(contig_length),
    avg_nt_id = mean(greatest_identity_percent),
    max_nt_id = max(greatest_identity_percent),
    min_nt_id = min(greatest_identity_percent)
    
  ) %>% 
  gt() %>% 
  cols_label(
    longest_contig = "Max Contig",
    shortest_contig = "Min Contig",
    avg_nt_id = "Average aa Id",
    max_nt_id = "Max aa Id",
    min_nt_id = "Min aa Id",
    num_contig = "n Contigs",
    num_species = "n Species"
  ) %>% 
  tab_header(
    title = paste0("Summary Stats for", " ", virus)
  ) %>% 
    fmt_number(
      columns = 4:last_col(),
      decimals = 2
    )

}
  
```



```{r, virus_profiles, results = 'asis'}
# Arrange df for virus reporting
virus_master_2023 <<- virus_master_2023 %>% 
  mutate(across(.cols = "virus_name",
                ~case_when(grepl("Manitoba", virus_name) & !grepl("Manitoba virus", virus_name) ~ 
                             paste0(., "*"),
                           TRUE ~ .))) %>% 
  arrange(desc(genome)) %>% 
  drop_na(virus_name)

for(virus in unique(virus_master_2023$virus_name)) {

  genome <- virus_master_2023 %>% 
    arrange(desc(genome)) %>% 
    drop_na(virus_name) %>%  
    filter(virus_name == virus) %>% 
    pull(genome) %>% 
    head(1)
  
  genome <- paste0(genome)
  
  family <- virus_master_2023 %>% 
    filter(virus_name == virus) %>% 
    pull(viral_family) %>% 
    head(1)
  
  family <- paste0(family)

  sample_detections <- virus_master_2023 %>% 
    filter(virus_name == virus) %>% 
    distinct(sample_number)
  sample_detections <- paste0(nrow(sample_detections))
  
  
  cat('\n\n#', virus, '\n\n')
  
  cat('\n\n>', virus, 'has a ', genome, 'genome and is a member of the family', family, 'and was detected in', sample_detections, 'sample(s).', 'See summary figures and tables below.',  '\n\n')
  
print(suppressMessages(
  virus_master_2023 %>% 
    filter(virus_name == virus) %>% 
    select(-"contig_sequence") %>% 
    group_by(mosquito_species, collection_year, location_pool) %>% 
    summarise(n = n_distinct(sample_number)) %>% 
    ggplot(aes(x = mosquito_species, y = n, fill = location_pool)) +
    geom_col() +
    facet_grid(. ~ collection_year) +
    scale_fill_viridis_d("Location", option = "magma") +
    theme_bw() +
    labs(title = paste0("Number of Virus Detections"),
       subtitle = paste0("For ", virus, " by Year, Species and Location")) +
    theme(plot.title = element_text(size = 16, face = "bold"),
        axis.title.x = element_blank(),
        axis.text.x = element_text(angle = 45, vjust = 0.5))
  ))
  
  cat('\n\n###', 'Years {.unlisted .unnumbered}', '\n\n')

  print(htmltools::tagList(
    virus_master_2023 %>% 
      filter(virus_name == virus) %>% 
      group_by(collection_year) %>% 
      summarise(n = n_distinct(sample_number)) %>% 
      gt() %>% 
      cols_label(collection_year = "Year(s) Detected")
    ))
  
  cat('\n\n###', 'Locations {.unlisted .unnumbered}', '\n\n')
  
    print(htmltools::tagList(
    virus_master_2023 %>% 
      filter(virus_name == virus) %>% 
      group_by(location_pool) %>% 
      summarise(n = n_distinct(sample_number)) %>% 
      gt() %>% 
      cols_label(location_pool = "Location(s) Detected")
    ))
    
    cat('\n\n###', 'Mosquito Species {.unlisted .unnumbered}', '\n\n')
  
    print(htmltools::tagList(
    virus_master_2023 %>% 
      filter(virus_name == virus) %>% 
      group_by(mosquito_species) %>% 
      summarise(n = n_distinct(sample_number)) %>% 
      gt() %>% 
      cols_label(mosquito_species = "Mosquito Hosts")
    ))
    
    cat('\n\n###', 'Contig Summary {.unlisted .unnumbered}', '\n\n')

  virus_profile_table(virus)
  
  print(htmltools::tagList(virus_table))
  
  cat('\n\n###', 'aa Identity Summary {.unlisted .unnumbered}', '\n\n')
  
  print(suppressMessages(
  virus_master_2023 %>% 
    filter(virus_name == virus) %>% 
    select(-"contig_sequence") %>% 
    ggplot(aes(x = greatest_identity_percent)) +
    geom_histogram(aes(fill = greatest_identity_percent < 90)) +
    geom_vline(xintercept = 90, linetype = "dotted", colour = "firebrick") +
    theme_bw() +
    labs(title = paste0("aa Identity"),
       subtitle = paste0("For ", virus),
       x = "aa Identity", 
       y = "Number of Contigs") +
    theme(plot.title = element_text(size = 16, face = "bold"),
        axis.title.x = element_blank(),
        axis.text.x = element_text(angle = 45, vjust = 0.5)) +
    theme(legend.position = "none")
  ))
  
}


```